Single-stranded DNA in tobacco leaf curl virus.
نویسندگان
چکیده
منابع مشابه
The capsid protein of tomato yellow leaf curl virus binds cooperatively to single-stranded DNA.
The capsid protein (CP) of tomato yellow leaf curl virus (TYLCV) is the only known component of the virus coat. Here, we identify TYLCV CP as a single-stranded (ss) DNA binding protein. Purified TYLCV CP bound ssDNA in a highly cooperative and sequence-nonspecific fashion. TYLCV CP-ssDNA complexes were resistant to nucleolytic digestion and remained stable at relatively high salt concentrations...
متن کاملNatural recombination between Tomato yellow leaf curl virus-is and Tomato leaf curl virus.
The complete genome sequences (2791 and 2793 nt) of isolates of Tomato yellow leaf curl virus-Is (TYLCV-Is) from Spain (SP72/97) and Portugal (Port2/95) were determined. These isolates are closely related to TYLCV-Is isolates reported in Japan (Japan-A and Japan-S) and Israel (Israel/Mild). Comparison of all sequenced isolates of TYLCV-Is showed that part of the genome comprising the intergenic...
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a begomovirus, tentatively named tobacco leaf curl japan virus-jpu (tblcjv-jpu), was isolated from lonicera japonica (honeysuckle) plants grown in utsunomiya showing veinal chlorosis symptoms. the tblcjv-jpu genome with 2,761 nt showed a highest identity with tblcjv-jp3 and was also close to tblcjv and as well to tblcjv-jp2. the overall nt identity with tblcjv-jp3 amounted to 92.94%, while the ...
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In South Asia, Cotton leaf curl disease (CLCuD) is caused by a complex of phylogenetically-related begomovirus species and a specific betasatellite, Cotton leaf curl Multan betasatellite (CLCuMuB). The post-transcriptional gene silencing (PTGS) suppression activities of the transcriptional activator protein (TrAP), C4, V2 and βC1 proteins encoded by Cotton leaf curl Kokhran virus (CLCuKoV)/CLCu...
متن کاملLigation of double-stranded and single-stranded [oligo(dT)] DNA by vaccinia virus DNA ligase.
Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed to either a poly(dA) or a poly(rA) backbone and, remarkably, free oligo(dT). Th...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1987
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.53.269